How to set up high-throughput synthesis at elevated temperature?

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We were looking for 96-well plates that can withstand temperatures over 100°C. Can anyone recommend any vendors?

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Helen Tran mentioned solutions like these to me a while ago. Courtesy of Google Lens, I came across V&P Scientific, Inc. See for example VP 416-ALB-96 | V & P Scientific, Inc. (also, [1] and [2])

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These ones enable a wider range of solvents and temperatures. There is probably a more traditional well plate with high temperature resistance though.

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We have printed well plates for an oven application using high temperature resin from Formlabs.

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That’s clever! We may do something similar in-house :blush:. cc @jeff.watchorn @yangcyril.cao @clascclon @nisfisker. For reference @shijing, here is Formlabs’ High Temp resin. You can also see the solvent compatibility, which seems pretty decent for High Temp resin. (aside: you can filter for desired properties using Powders and Resins for 3D Printing | Formlabs, which shows that Rigid 10K is also suitable for elevated temperatures).

@Lilo do you remember who had the CAD file for this? I can ping to ask. It doesn’t seem like any files are available from the Opentrons Labware Library. Of course, there are designs out there like https://grabcad.com/library/96-well-plate-flat-bottom-standard-1 from GrabCAD.

I’m giving the one from above a go with draft resin (EDIT: this orientation made it hard to remove the supports and resulted in damage to some of the wells):

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It looks like you can also purchase custom parts using Formlabs High Temp resin from Protolabs Network (xref: prototyping and Where do you buy things? (custom parts, off-the-shelf, internal tools, etc.) - #2 by sgbaird).

It was fairly straightforward to get the quote (SLA printing → upload STL → edit settings and select Formlabs High Temp). The part quote was for 166.18 USD. Note that the model above shows 126.13 mL as the material usage, and this equates to ~25 USD (199 USD per liter of High Temp resin) if you were to print it yourself, though you also have to do the post-processing (wash, removal of supports, and cure) on your own.

STL file (from GrabCAD): 96 Well Plate, F-bottom, Standard - 96 Well Plate, F-bottom, Standard.stl (6.0 MB)

Formlabs file: 96-well-plate.form (6.5 MB)

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For these I’m more interested in engineering PTFE?

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These are some options that might work. I’m not sure about using 3D printed ones as they may be difficult to clean as the surface isn’t completely smooth.

  1. Quartz plates: General Standard Quartz GLass UV 96 Well Plates Sigma / 96 well microp – MICQstore)
  2. You could machine something out of aluminum.
  3. Could try a polypropylene plate depending on the temperature (up to about 121 degC). They do not have the flat bottoms traditional multiwell plates have. (Thermo Scientific™ Nunc™ 96-Well Polypropylene Sample Processing & Storage Microplates | Fisher Scientific)
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